study on hematological parameters and serum enzymes in common carp (cyprinus carpio ) after low cadmium concentration exposure

نویسندگان

سید سعید میرزرگر

فرزاد غیاثی

حسینعلی ابراهیم زاده موسوی

جمیله سالار عاملی

علیرضا باهنر

چکیده

the 60 apparently common carp, cyprinus carpio (mean weight 700g.) divided to two groups (1:control and 2: test) and transferred to 1000 liter indoor fiberglass tanks supplied with static well water, continuous aeration and defined water quality. test group was exposed to 30ppb (low concentration) cadmium as cdcl 2 (merck) control group was without cadmium addition . blood samples were caught randomly from 5 fish in each group with duplicate, by using exsanguination of caudal vein at the day 15 and 30 after exposure. the hematological parameters such as: wbc , rbc, differential count, hematocrit - total protein , albumin , and globulin concentrations , the albumin- globulin ratio and levels of serum enzymes such as: ldh , alt , ast and alp were monitored. the results revealed that white blood cell (wbc: x 104 /µl) count decreased significantly (p< 0.05) from 3.98 ± 1.32 in group 1 (control , non-exposed to cadmium) to 2.52 ± 0.3 in group 2 (test , exposed to cadmium) on the 15th day post exposure. also , the wbc count significantly (p < 0.05)decreased in test group from 2.52 ± 0.3 on the 15th day post exposure to 1.7±0.33 on the 30th day post exposure. based on results the level of ldh enzyme (u/l) after 30 days exposure , in control group 430±69.35 significantly (p

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